Cat. No.
MABL-589
Application
functional assay, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Cow
Clone No.
4B10-A3-E4
From
Recombinant Antibody
Specificity
This antibody is specific for calf thymus ATPase A and recognizes bovine, human, and mouse homologs. Calf ATPaseA is a TP-dependent annealing helicase that binds selectively to fork DNA relative to ssDNA or dsDNA and catalyzes the rewinding of the stably unwound DNA. The antibody also shows a reaction to the denatured protein.
Alternative Names
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1; HepA-related protein; Sucrose nonfermenting protein 2-like 1
UniProt
Q9TTA5
Immunogen
The mouse version of this antibody was generated by immunizing mice with immunopurified calf thymus ATPaseA.
Application Notes
The mouse version of this antibody was used for a western blot on calf DNA- dependent ATPase A isolated from calf thymus using immunoprecipitation (Mesner et al, 1993; pmid:8394114). To identify the mouse version of this antibody an ELISA on native ATPase A was preformed. Furthermore, a western blot was preformed on bacteriophage T4 gene 44/62 and 45 proteins. It showed a strong reaction (Mesner et al, 1991; pmid:1720977). The ATPase A activity under either Ca2+-activated or Mg3+-actin-activated conditions were determined in the presence of this antibody. No change in activity was noticed (Eldin et al, 1993; pmid:8448113).
Antibody First Published
Mesner et al. DNA-dependent adenosinetriphosphatase A is the eukaryotic analogue of the bacteriophage T4 gene 44 protein: immunological identity of DNA replication-associated ATPases Biochemistry. 1991 Dec 10;30(49):11490-4. PMID:1720977
Note on publication
The construction of three stable murine hybridomas that secrete monoclonal antibodies which recognize calf thymus DNA-dependent adenosinetriphosphatase A is reported. All three of the antibodies react specifically with calf thymus ATPase A and the gene 44 protein from the bacteriophage T4 DNA-dependent ATPase.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
