Cat. No.
MABL-599
Application
WB, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
4F6
From
Recombinant Antibody
Specificity
This antibody is specific for the photoconverted form of CaMPARI-Red (both CaMPARI1 and CaMPARI2). CaMPARI is s a photoconvertible protein construct that allows imaging of the integrated calcium activity of populations of cells over time.
Alternative Names
CaMPARI 1&2; CaMPARI1; CaMPARI2; CaMPARI-Red; Calcium Modulated Photoactivatable Ratiometric Integrator; 4F6-1
UniProt
Immunogen
This antibody was originally raised by immunizing mice with a purified and photoconverted EosFP protein, subjected it to proteolysis, and further purified the proteolytic fragment containing the red chromophore which is identical to the equivalent region of CaMPARI1 and CaMPARI2.
Application Notes
4F6 is recommended for the detection and analysis of CaMPARI via immunohistochemistry (IHC) and Western blot (WB) (Moeyaert et al., 2018; PMID: 30361563). For instance, it was successfully used in the immunohistochemical identification of CaMPARI-Red cells in brain tissue, distinguishing them from the cells in which CaMPARI stayed in ints green, unconverted form (Ebner et al., 2019; PMID: 31178713). This product (Ab01649-23.0) was used for indirect immunofluorescence (IF) staining of adult female Drosophila fly brains to visualize and detect the localization of the CaMPARI-Red protein (Gendron et al., 2023; PMID: 37310911).
Antibody First Published
Moeyaert et al. Improved methods for marking active neuron populations Nat Commun. 2018 Oct 25;9(1):4440. doi: 10.1038/s41467-018-06935-2 PMID:30361563
Note on publication
The original publication presents the development of CaMPARI2, an improved fluorescent protein sensor with enhanced properties for marking active neuronal populations, along with the introduction of an immunohistochemical detection method using an anti-CaMPARI-red antibody for selective labeling of photoconverted CaMPARI in activated neurons in rodent brain tissue.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
