Cat. No.
MABL-601
Application
cryoEM, crystallography, HI, NTRL, RIA, ELISA
Isotype
Engineer antibody
Species Reactivity
canine parvovirus
Clone No.
Mab14
From
Recombinant Antibody
Specificity
This antibody is specific for Site A of CPV-2's capsid.
Alternative Names
Coat protein VP1; CPV-2; Fab 14; Antibody 14; ABCD_AD535
UniProt
P17455
Immunogen
The original antibody was generated by immunizing BALB/c mice with purified Capsids of CPV and FPV which were prepared from infected feline cells.
Application Notes
The original version of this antibody (IgG2a) was raised and tested against CPA, FPV, and MEV and was found to inhibit and neutralize CPV-a and -b in neutralization and HI assays (Parrish et al., 1982; PMID: 6180709). The original version of this antibody was used in RIA and HI assays. In the HI test, it was found to inhibit CPV-a;-b;-c-;-d; and -e, and in the RIA, it was found to react with CPV-d variants 6, 12, and 8 (Parrish and Carmichael, 1983; PMID: 6194613). The Fab version of this antibody was used in a cryoEM analysis of capsid: Fab complexes, and its Kd was measured via ELISA as 3.4 × 10-9 M (Nelson et al., 2007; PMID: 17217977). The Fab version of this antibody was crystallized, and its structaure was determined (Hafenstein et al., 2009; PMID: 19321620).
Antibody First Published
Parrish et al. Antigenic relationships between canine parvovirus type 2, feline panleukopenia virus and mink enteritis virus using conventional antisera and monoclonal antibodies. Arch Virol. 1982;72(4):267-78. doi: 10.1007/BF01315223. PMID:6180709
Note on publication
The original publication investigates the antigenic relationships between canine parvovirus type 2 (CPV), feline panleukopenia virus (FPV), and mink enteritis virus (MEV) using conventional antisera and monoclonal antibodies.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
