Cat. No.
MABL-611
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Burkholderia mallei, Burkholderia pseudomallei
Clone No.
P7F4
From
Recombinant Antibody
Specificity
This antibody binds surface capsular antigens of Burkholderia pseudomallei (BP), a causative agent of melioidosis, is a gram-negative, facultative anaerobic, motile bacillus commonly found in the soil and stagnant waters. BP infection is often due to either direct inoculation into wounds and skin abrasions or inhalation of contaminated materials. Recently, BP has been recognized as a major cause of community- acquired septicemia, resulting in significant mortality. This antibody also cross reacts with surface capsular antigens of Burkholderia mallei. B. mallei (BM), a host-adapted pathogen that does not normally persist in nature, causes glanders in horse. Some studies indicated that BM is highly infectious in humans by aerosol route.
Alternative Names
surface capsule antigen; Burkholderia mallei; BM; glanders; Burkholderia pseudomallei; BP; Burkholderia spp; melioidosis; Whitmore’s disease
UniProt
Immunogen
The original antibody was generated by immunizing mice with various antigen preparations of B. pseudomallei (ATCC 23343). Later on phage display technique was used to generate scFv version of this antibody.
Application Notes
This antibody can be used for the detection of capsular antigens of Burkholderia pseudomallei and Burkholderia mallei in a sample using. The binding affinity of the antibody against B. pseudomallei was measured (0.449 at OD405nm) (Kim et al., 2011; PMID: 21172353).
Antibody First Published
Kim et al. Construction and molecular characterization of mouse single- chain variable fragment antibodies against Burkholderia mallei and Burkholderia pseudomallei. J Immunol Methods. 2011 Feb 28;365(1-2):101-9. PMID:21172353
Note on publication
The paper describes the molecular characterization of scFv antibodies against Burkholderia mallei and Burkholderia pseudomallei generated using phage display technology.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
