Cat. No.
MABL-621
Application
IP, WB, FC, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
AC6C3
From
Recombinant Antibody
Specificity
This antibody can recognize antigens expressed on the cell surface of human ovarian, cervix, breast, colon, melanoma and other carcinomas. This antibody does not react with normal human tissues. The epitope recognized by this antibody is different from the one recognized by another similar antibody CR4E8.
Alternative Names
cancerous cells
UniProt
Immunogen
The original antibody was generated by fusing regional lymph node lymphocytes from a patient with ovarian carcinoma with cells of the hybrid myeloma line SPAZ4.
Application Notes
This antibody immunoprecipitated a 32 kD polypeptide from lysates of the ovarian tumor cell line SKOV3. Cell surface immunofluorescence determinations using the fluorescence activated cell sorter revealed that this antibody stain ovarian or cervix carcinoma tumor cell lines (PMID： 7756578). Immunoperoxidase staining with this antibody of nonpermeabilized cryostat sections of freshly obtained or cryopreserved ovarian carcinoma specimens and human ovarian tumor xenografts demonstrated strong reactivity of these specimens. This antibody mediated complement-dependent cytotoxicity and identified a 32 Kd band in western blotting and immunoprecipitation experiments conducted on surface labelled SKOV3 cells. The association constant for the IgM version of the antibody was determined at 2.3 x 10^(10) M^(-1) (PMID： 2032733). The biodistribution of 111In-labeled IgM conjugate of this antibody was studied after i.v. or i.p. administration in nude mice bearing an s.c. xenograft or peritoneal tumor lumps of a human colorectal carcinoma (SW620). The i.p. administration of intact, specific radiolabeled IgM provides prolonged retention of radioactivity in tumor, low normal tissue uptake, a long peritoneal residence time, and very limited spillover of IgM into the circulation (PMID：7493338).
Antibody First Published
Freedman et al. Development of a cell surface reacting human monoclonal antibody recognizing ovarian and certain other malignancies. Hybridoma. 1991 Feb;10(1)：21-33. PMID:2032733
Note on publication
Describes the generation of two antibodies against different antigens present on carcinomas.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
