Cat. No.
MABL-620
Application
in vivo, RIA, therapeutic, WB, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
COL-1
From
Recombinant Antibody
Specificity
The antibody is specific for CEA. COL-1 does not cross-react with NCA, NFA-1, BGP and PMNs. CEA is one of the most commonly used tumor markers in serum immunoassay determinations of carcinoma.
Alternative Names
CEA
UniProt
Q13984
Immunogen
The original antibody was generated by immunizing BALB/c mice with extracts or membrane- enriched fractions of biopsy material from either primary or metastatic colon carcinoma lesions.
Application Notes
The antibody reacted with purified carcinoembryonic antigen in solid-phase radioimmunoassay and by Western Blot analysis. The antibody was tested by fluorescence-activated cell sorting for cell surface binding to the LS174T WiDr and HT-29 colon carcinoma cell lines. The antibody was tested by FACS for binding to the surface of normal human polymorphonuclear leukocytes where it appeared nonreactive (Muraro et al., 1985; PMID: 2413997). The antibody was shown to bind CEA with a Ka of 1.4 x 10^9 M^-1 (Kuroki et al., 1989; PMID: 2474516). The antibody was used for immunohistochemistry on colorectal, gastric, and lung adenocarcinoma tumor tissues. In addition, pancreatic, breast, gallbladder, bile duct, malignant ovarian teratoma, mucinous endometrial carcinoma, colon cancer, remote colon mucosa and bladder cancer stained positive (Shi et al., 1994; PMID: 7520463 and Robbins et al., 1993; PMID: 8386136). The antibody reacted by immunofluorescence with a mouse colon adenocarcinoma MC-38 expressing high levels of cell-surface CEA cell line, but not with the NCA or BGP transfectants (Robbins et al., 1993; PMID： 8386136). 125I-labeled COL-1 IgG was shown to efficiently and specifically target the LS- 174T human colon carcinoma xenograft in athymic mice. 131I-labeled COL-1 demonstrated a reduction of tumor growth rate in the same model (Siler et al., PMID： 8292968). I131-labeled COL-1 was tested in phase I trial in patients with gastrointestinal malignancies (Yu et al. 1996). The humanized version of the antibody was constructed and used in the detection and treatment of a CEA-expressing tumor (US8828717B2).
Antibody First Published
Muraro et al. Definition by monoclonal antibodies of a repertoire of epitopes on carcinoembryonic antigen differentially expressed in human colon carcinomas versus normal adult tissues Cancer Res. 1985 Nov;45(11 Pt 2)：5769-80. PMID：2413997
Note on publication
The original paper describes the generation and characterization of a set of antibodies. The antibodies show selective reactivity for human colon carcinomas.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
