Cat. No.
MABL-619
Application
Blocking, crystallography, IB, ITC, WB, ELISA, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
M75
From
Recombinant Antibody
Specificity
This antibody is specific for the sequence PGEEDLP, which is repeated several times between the 61st and 96th aa residues of human carbonic anhydrase IX's PG domain.
Alternative Names
CA9; CAIX; MN/CA IX; endogenous protein p54/58N
UniProt
Q16790
Immunogen
The original antibody was generated by immunizing BALB/C mice with MaTu-infected HeLa cells.
Application Notes
The original format of this antibody (mouse IgG2b) was characterized by and used in IB (WB), IF, and FC assays (Pastoreková et al., 1992; PMID: 1312272). This antibody's epitope has been determined by a competitive ELISA against the peptide APGEEDLPA. Furthermore, it was used for WB and was shown to blocks the capacity of immobilized MN/CA IX to attach cells (Závada et al., 2000; PMID: 10839295). The Fab version of this antibody was crystallized and analyzed. Additionally, an scFv version of this antibody was created, and its thermodynamic parameters against the peptide DLPGEEDLPGEE were evaluated via ITC. Its Kd was determined to be 0.42 μM (Král et al., 2008; PMID: 18041760). This antibody was used for WB in breast cell lines and for IHC in xenograft tumors grown from PC-3 cells in mice (Li et al.,2009; PMID: 19538935).
Antibody First Published
Pastoreková et al. A novel-quasi-viral agent, MaTu, is a two-component system. Virology. 1992 Apr;187(2):620-6. PMID:1312272
Note on publication
The original publication introduces MaTu, a quasi-viral agent derived from a human mammary tumor, and details the generation and characterization of monoclonal antibodies against it.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
