Anti-CD147 mAbs [5F6](MABL-722)

MABL-722

Blocking, crystallization, in vivo, inhibition, ELISA

Engineer antibody

Human

5F6

Recombinant Antibody

The antibody binds the N domain of CD147. The antibody binds to an epitope on the extracellular domain of CD147 (residues Asp65-Phe74 and Val30-Thr40). CD147 is a type I membrane glycoprotein and is classified into the immunoglobulin-like superfamily, and is expressed in many types of cancer. It is suggested that CD147 is also associated with cancer metastasis, invasion, and chemical drug resistance.

Basigin; Emmprin; 5F7; Collagenase stimulatory factor; Extracellular matrix metalloproteinase inducer; Hepatoma-associated antigen; HAb18G; Leukocyte activation antigen M6; OK blood group antigen; Tumor cell-derived collagenase stimulatory factor; TCSF;BSG; Cluster of differentiation 147

P35613

The original antibody was generated by immunizing mice with CD147.

The specificity of the antibody was coonfirmed by competition ELISA. The antibody recognized MDA-MB-231 cells that express native CD147 antigens on tumor cell surface. To assess the ability of the original antibody to block MMP-1 production, NHLF were treated with recombinant a ECD-Flag construct and the MMP-1 production in response to CD147 stimulation was determined using the MMP-1 activity assay. The antibody showed significant inhibition of MMP-1 production. The original antibody was screened for the ability to inhibit CD147-induced MMP-2 or MMP-9 production in co-culture. Results showed the antibody inhibited MMP-2 and MMP-9 production in co-culture. The humanized version of the antibody (IgG1) inhibited CD147-stimulated MMP-1 production by fibroblast in a dose-dependent manner and the MMP-2 production by MDA-MB-231 and NHDF in a dose dependent manner. The humanized version of the antibody was able to inhibit VEGF production by normal human lung fibroblasts (NHLF) stimulated with 10 μg/ml recombinant CD147 for 48 hours. The humanized version of the antibody inhibited more than 40% of VEGF production in co-culture of tumor cells and fibroblast cells. The humanized version of the antibody inhibited angiogenesis in vivo. SCID Beige Mice implanted with MDA-MB-231 human breast carcinoma cells were treated with E+5F6(MuIgG2a); the antibody inhibited tumor angiogenesis in Panc-1 matrigel plug model. The antibody showed potential as therapeutic and prophylactic agents (US8618264B2). The structure of the Fab fragment was determined (Teplyakov et al., 2010).

100 μg Purified antibody.

1 mg/ml.

Protein A affinity purified

PBS with 0.02% Proclin 300.

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.