Cat. No.
MABL-723
Application
IP, WB, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
OX-114
From
Recombinant Antibody
Specificity
OX-114 binds to both the 2- and 3-domain isoforms of CD147. CD147 targets monocarboxylate transporters to the plasma membrane, and is involved in spermatogenesis, embryo implantation, neural network fomration and tumor progression. Stimulates production of matrix metalloproteases. Possibly a receptor for oligomannosidic glycans. CD147 found only in vascular endothelium in non-neoplastic regions of the brain whereas it is present in tmour cells but not in proliferating blood vessels in malignant gliomas.
Alternative Names
Basigin; basic immunoglobulin superfamily; HT7 antigen; membrane glycoprotein gp42; gp42; gp-42; OX114; MRC OX-114; MRC OX114
UniProt
P18572
Immunogen
OX-114 was generated by immunizing rats with recombinant mCD147d0-2rCD4d3+4.
Application Notes
This antibody binds mouse CD147, and has been used in FC and IHC-fr analysis to characterise CD147 and detect the cell types on which it is expressed, indicating that CD147 is a lymphocyte activation marker (Paterson, 1987). This antibody failed to give immunoprecipitates from radiolabelled activated T cells.
Antibody First Published
Hanna et al. A novel form of the membrane protein CD147 that contains an extra Ig-like domain and interacts homophilically. BMC Biochem 4:17 2003 PMID:14606962
Note on publication
Describes the original use of this antibody as part of FC and IP studies to identify new markers for activated T cells.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
