Cat. No.
MABL-728
Application
IP, WB, ELISA, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
11G5a
From
Recombinant Antibody
Specificity
This antibody binds to the CD151-integrin α3β1 binding site of the CD151 extracellular domain.
Alternative Names
Tetraspanin-24; GP27; MER2; Platelet-endothelial tetraspan antigen 3; PETA-3; RAPH; SFA1; TSPAN24; Cluster of Differentiation 151
UniProt
P48509
Immunogen
This antibody was raised by immunizing mice.
Application Notes
The antibody has been used in flow cytometry experiments and to assess the interaction of CD151 with integrins (Yauch 1998). CD151 can also regulate cell migration independent of integrins in cancer for tumour cell migration, and mAb 11G5a has been used for histologic detection of CD151 free in prostate cancer, which correlates with poor patient outcome (TD Palmer 2014).
Antibody First Published
Yauch et al. Highly Stoichiometric, Stable, and Specific Association of Integrin α3β1 with CD151 Provides a Major Link to Phosphatidylinositol 4-Kinase, and May Regulate Cell Migration Mol Biol Cell. 1998 Oct; 9(10)： 2751–2765 PMID：9763442
Note on publication
Describes the Specific Association of Integrin α3β1 with CD151 and its link to cell migration
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
