Cat. No.
MABL-727
Application
IP, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
BRA-4F1
From
Recombinant Antibody
Specificity
This antibody is specific for CD15 (Appelmelk. et al, 1996, pmid：8675304). It also shows crossreactivity with the CD15 antigen from other species.
Alternative Names
sLeX; Lewis X antigen; Leu-M1; Sialyl-Lewis X; SSEA-1; sialyl LeX
UniProt
Immunogen
This antibody was raised by immunzing mice with human CD15.
Application Notes
This antibody has been used in flow cytometry (Appelmelk. et al, 1996). The antibody has been shown to be usefull in immunohistochemistry (Appelmelk. et al, 1996). The antibody is utilized in immunopercipitation (Akin. et al, 2000). Immunofluorescence is a proven application of this antibody (Vilella. et al, 2003). CD15 is expressed in Reed-Sternberg cells, which are a sign of Hodgkin's lymphoma, and thus the antibody can be used in immunohistochemistry to detect this kind of cancer (Trillo-Tinoco. et al, 2019). Finally, BRA-4F1 can be used in fertility research as the CD15 antigen is a molecule that is nessecary for a sperm cell to recognize and fertilize an egg cell.
Antibody First Published
Appelmelk. et al Potential Role of Molecular Mimicry between Helicobacter pylori Lipopolysaccharide and Host Lewis Blood Group Antigens in Autoimmunity INFECTION AND IMMUNITY, June 1996, p. 2031–2040 PMID：8675304
Note on publication
The article describes the binding of the antibody to CD15 compared to another anti- CD15 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
