Cat. No.
MABL-844
Application
inhibition, WB, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
P4A8
From
Recombinant Antibody
Specificity
This antibody is specific for human Fn14.
Alternative Names
FN14; TweakR; TNFRSF12A; FGF-inducible 14; Tweak-receptor; Tumor necrosis factor receptor superfamily member 12A; Fibroblast growth factor-inducible immediate-early response protein 14
UniProt
Q9NP84
Immunogen
The original antibody was raised by immunizing Fn14-deficient mice with CHO cells expressing human surface Fn14 and boosted with Fn14-myc-His
Application Notes
The original antibody was tested to inhibit distinct TWEAK-induced cellular effects known to be mediated by Fn14. HT29 and WiDr cells were treated with this antibody, this resulted in a strong inhibition of TWEAK-induced IL8 production. Furthermore, it was shown that oligomerization can greatly increase the agonistic activity of this antibody (Salzmann et al., 2013; PMID：23532848). This antibody was used for flow cytometry on HEK293 cells transfected with plasmids expressing Fn14 protein. This antibody was used for a western blot on lysates from HEK293 cells expressing Fn14 (Trebing et al., 2014; PMID：24135629). This antibody was used for immunohistochemistry on different human brain tumors (Tran et al., 2006; PMID：17018610), and on samples from xenografted tumors made from H460 cells (Jandova et al., 2015; PMID：25563366).
Antibody First Published
Tran et al. Increased fibroblast growth factor-inducible 14 expression levels promote glioma cell invasion via Rac1 and nuclear factor-kappaB and correlate with poor patient outcome Cancer Res. 2006 Oct 1;66(19)：9535-42. PMID：17018610
Note on publication
The original paper shows that the fibroblast growth factor-inducible 14 (Fn14), a member of the tumor necrosis factor (TNF) receptor superfamily, is expressed at high levels in migrating glioma cells in vitro and invading glioma cells in vivo.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
