Cat. No.
MABL-859
Application
CoStim, Superagonism, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Rat
Clone No.
JJ316
From
Recombinant Antibody
Specificity
Recognises rat CD28 and can trigger T-cell proliferation in vitro and in vivo.
Alternative Names
TP44; T-cell-specific surface glycoprotein CD28
UniProt
P31042
Immunogen
Made by immunizing BALB/c mice with rat CD28 cDNA-transfected A20/J cells.
Application Notes
This antibody binds rat CD28 and can convey as a co-stimulatory signal. At 100-1000- fold higher concentration than required for co-stimulation JJ316 (unlike the clone JJ319 marketed by competitors (see Tacke et al. 1997)) can induce proliferation of all T-cell subsets without TCR engagement.
Antibody First Published
Tacke et al. CD28-mediated induction of proliferation in resting T cells in vitro and in vivo without engagement of the T cell receptor: evidence for functionally distinct forms of CD28. Eur J Immunol PMID:9022025
Note on publication
Describes the in vivo and in vitro characterisation of JJ316.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
