Cat. No.
MABL-866
Application
in vitro, FC
Isotype
Engineer antibody
Species Reactivity
Human, monkey
Clone No.
CD3B219
From
Recombinant Antibody
Specificity
The antibody is specific for CD3 epsilon.
Alternative Names
T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e; SP34-2
UniProt
P07766
Immunogen
Application Notes
The antibody was used to construct different CD33 xCD3 bispecific antibodies (IgG4). In vitro T cell mediated cytotoxicity assays were performed using the bispecific antibody and the cytotoxicity was measured by flow cytometry. Ex vivo cytotoxicity assay showed the paired antibody promoted a dose-dependent reduction of total cytotoxicity that correlated with T cell activation after 48 hours (US20210040209A1). The antibody is considered a high affinity binder (Kd= 8mM) (US20210214440A1). The CD33×CD3 bispecific antibodies specifically induce cell cytotoxicity of MOLM-13 cells and CD33+ monocytes at 48 hr (US20200048349A1).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
