Cat. No.
MABL-867
Application
Activate, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
12F6
From
Recombinant Antibody
Specificity
This antibody binds to human CD3.
Alternative Names
Leu4/T3; ET-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e
UniProt
P07766
Immunogen
Human CD3
Application Notes
This antibody competes for CD3 binding with the therapeutic antibody OKT3 (Ab00122). 12F6 binding to CD3 on human T-cells stimulates the secretion of TNF-alpha, INF-gamma and IL-10. Humanized versions of this antibody are also available.
Antibody First Published
Wong & Colvin Bi-specific monoclonal antibodies: selective binding and complement fixation to cells that express two different surface antigens. J Immunol. 1987 Aug 15;139(4):1369-74. PMID:3112233
Note on publication
Describes generation of anti-CD3 antibody and further use in generation of bi- specific hybrid-hybridoma.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
