Cat. No.
MABL-960
Application
IP, WB
Isotype
Engineer antibody
Species Reactivity
Rat
Clone No.
OX-56
From
Recombinant Antibody
Specificity
OX-56 binds specifically to CD43 and binding is not affected by CD43 glycosylation. OX-56 reacts with most thymocytes and T lymphocytes in the lymph node cell population. This antibody also binds to pre-B cells and can detect CD43 at low levels on B cells. CD43 plays a role in the physicochemical properties of the T-cell surface and in lectin binding. CD43 is a major cell-surface sialoglycoprotein - also found on neutrophils, bone marrow stem cells, B-lineage cells after activation of thymocytes and T lymphocytes but is not expressed on resting B lymphocytes. The protein presents carbohydrate ligands to selectins and Is a counter-receptor for SN/Siglec-1. During T-cell activation it is actively removed from the T- cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response.
Alternative Names
Leukosialin; OX56; MRC OX-56; MRC OX56; sialophorin
UniProt
P13838
Immunogen
OX-56 was prepared by immunizing BALB/c mice with activated rat T lymphocytes.
Application Notes
OX-56 has been used in the purification of rat leukosialin by immunoaffinty chromatography (Willis et al. 1987) as well as in WB.
Antibody First Published
Cyster et al The dimensions of the T lymphocyte glycoprotein leukosialin and identification of linear protein epitopes that can be modified by glycosylation. EMBO J. 1991 Apr;10(4):893-902. PMID:1706994
Note on publication
Describes the use of OX-56 in the Epitope mapping of leukosialin
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
