Cat. No.
MABL-961
Application
RIA, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Rat
Clone No.
W3/13
From
Recombinant Antibody
Specificity
This antibody binds to CD43, which is expressed on lymphocytes.
Alternative Names
Leukosialin; sialophorin; Leukocyte sialoglycoprotein; Ly-48; Ly48
UniProt
P13838
Immunogen
Rat thymocyte membrane glycoproteins injected into BALB/c mice.
Application Notes
This antibody detects CD43 on lymphoid cells, and can be used to label thymocytes, lymphocytes from the bone marrow and thoracic duct lymphocytes (Williams et al, 1977). Similarly, W3/13 can be used in immunohistochemical staining, enabling the elucidation of the cellular organisation of lymph nodes (Barclay, 1981).
Antibody First Published
Williams et al. Analysis of cell surfaces by xenogeneic myeloma-hybrid antibodies: Differentiation antigens of rat lymphocytes Cell, 1977, Vol.12(3), pp.663-673 PMID:303545
Note on publication
Describes the original use of this antibody to detect CD43 in lymphoid cell labelling studies
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
