Cat. No.
MABL-964
Application
WB, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
156-3C11
From
Recombinant Antibody
Specificity
This antibody is specific for human CD44.
Alternative Names
CDw44; PGP-1; PGP-I; ECMR-III; Epican; HUTCH-I; Hermes antigen; Hyaluronate receptor; Phagocytic glycoprotein I; Phagocytic glycoprotein 1; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor
UniProt
P16070
Immunogen
The original antibody was raised by immunzing a mouse with human CD44.
Application Notes
The original antibody was used for immunofluorescence on HeLa cells (Pleiner et al., 2018; PMID:29263082), and on PC3 cells (Ricciardelli et al., 2007; PMID:17293599). This antibody was used for flow cytometry on CAMA-1, MDA-MB-231, MDA-MB-435, Raji and HeLa cells (Ssadh et al., 2017; PMID:29190904), and on HEK 293T human embryonic kidney cells, MDA-MB231 human breast tumor cells, MCF-7 human breast tumor cells, 4T1 mouse breast tumor cells, and Panc-1 human pancreatic tumor cells (Khan et al., 2021; PMID:33391537), to determine the expression of CD44 on their surface. This antibody was used for for an immunoblot on CAMA-1, MDA-MB-231, MDA-MB-435, Raji and HeLa cells to confirm that the receptor detected by tha antibody was indeed CD44 (Ssadh et al., 2017; PMID:29190904). This antibody was used for immunohistochemistry to visualize CD44 in human testicular tissues (Perri et al., 2022), in human salivary gland mucoepidermoid carcinoma and HMC cell lines (Andrews et al., 2019;PMID:30498096), and on pericellular sheath, prostate cancer cells (Ricciardelli et al., 2007; PMID:17293599).
Antibody First Published
Ricciardelli et al. Formation of hyaluronan- and versican-rich pericellular matrix by prostate cancer cells promotes cell motility J Biol Chem. 2007 Apr 6;282(14):10814-25.PMID:17293599
Note on publication
In the original paper a particle exclusion assay was used to determine whether human prostate cancer cell lines are capable of assembling a pericellular sheath following treatment with versican-containing medium and whether formation of a pericellular sheath modulated cell motility.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
