Cat. No.
MABL-989
Application
Blocking, FC, IHC-Fr
Isotype
Engineer antibody
Species Reactivity
Rat
Clone No.
OX-30
From
Recombinant Antibody
Specificity
This antibody recognizes all molecular forms of rat L-CA, and is noncompetitive with OX-1, 28 and 29 in binding to L-CA. While purified L-CA inhibited binding of this antibody to lymph node cells, no such inhibition of binding was seen with reduced and alkylated L-CA.
Alternative Names
PTPRC; B220; CD45; CD45 R; GP180; L-CA; LCA; LY5; T200; protein tyrosine phosphatase; receptor type C; Receptor-type tyrosine-protein phosphatase C
UniProt
P04157
Immunogen
This antibody was prepared by immunizing BALB/c mice with rat lymph node glycoproteins.
Application Notes
This antibody binds all molecular forms of rat L-CA, and consequently can be used to label thymocytes, T cells, B cells and most bone marrow cells (Woollett, 1985).
Antibody First Published
Woollett et al. Molecular and antigenic heterogeneity of the rat leukocyte- common antigen from thymocytes and T and B lymphocytes. Eur J Immunol. 1985 Feb;15(2):168-73.PMID:2578966
Note on publication
Describes the original use of this antibody in FC and blocking analysis to assess the molecular and antigenic heterogenity of rat L-CA.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
