Cat. No.
MABL-1037
Application
IHC-P, IP, FC, IHC-Fr
Isotype
Engineer antibody
Species Reactivity
Rat
Clone No.
OX-44
From
Recombinant Antibody
Specificity
This antibody recognises CD53, which is expressed on peripheral T-cells, single (CD4 or CD8) positive thymocytes, B-cells, monocytes, macrophages, neutrophils, dendritic cells and microglial cells. This antibody recognises a conformational epitope that is dependent on disulphide bonding.
Alternative Names
Leukocyte surface antigen CD53; Cell surface glycoprotein CD53; Leukocyte antigen MRC OX-44
UniProt
P24485
Immunogen
This antibody was prepared by immunizing BALB/c mice with T lymphoblasts from an MLR between purified rat T helper cells and irradiated semiallogeneic rat spleen.
Application Notes
This antibody binds rat CD53, and consequently labels all myeloid cells and peripheral lymphoid cells, but only 12% of thymocytes (Paterson, 1987). Functional studies indicate that this subset of OX-44+ thymic cells includes all thymocytes that are on the pathway to immunocompetent T lymphocytes. By contrast, the OX-44- compartment represents cells that are negative for proliferation and thymopoiesic activity, and are fated to die in situ. In addition, this antibody has been used in conjunction with CD4 and CD8 antigens to define seven subsets of thymic cell. Most CD4-CD8- cells are OX-44+, whereas almost all CD4+ CD8+ cells were OX-44-
Antibody First Published
Paterson et al. The MRC OX-44 antigen marks a functionally relevant subset among rat thymocytes. J Exp Med. 1987 Jan 1;165(1):1-13. PMID:3098892
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
