Cat. No.
MABL-1118
Application
FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Primates, Human
Clone No.
BU88
From
Recombinant Antibody
Specificity
This antibody is specific for CD8. It is not specific to humans
Alternative Names
CD8a; T-lymphocyte differentiation antigen T8/Leu-2; T-cell surface glycoprotein CD8 alpha chain
UniProt
P01732
Immunogen
The original antibody was raised by immunizing a mouse with human CD8.
Application Notes
The original antibody was used for flow cytometry on blood lymphocytes (Arab et al., 2015; PMID:25926938, Langermans et al., 2000; PMID:11168830), and on PMBCs form chimpanzees, rhesus macaques and squirrel monkeys (Ozwara et al., 1997; PMID:9379483). The antibody was used for immunofluorescence on human PBMCs (Langermans et al., 2000; PMID:11168830). This antibody was used for immunohistochemistry on paraffin embedded tumor sections (Lin et al., 2020; PMID:31690181).
Antibody First Published
Ozwara et al. Flow cytometric analysis on reactivity of human T lymphocyte- specific and cytokine-receptorspecific antibodies with peripheral blood mononuclear cells of chimpanzee (Pan troglodytes), rhesus macaque (Macaca mulatta), and squirrel monkey (Saimiri sciureus) J Med Primatol. 1997 Jun;26(3):164-71. PMID:9379483
Note on publication
In the original paper they identified a large number of mAb that can be used in future immunological studies in three different nonhuman primates, i.e., chimpanzees, rhesus macaques, and squirrel monkeys.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
