Cat. No.
MABL-1141
Application
IP, WB, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
BU63
From
Recombinant Antibody
Specificity
This antibody binds CD86, which binds CD28 on the surface of T cells and is responsible for their activation during an adaptive immune response.
Alternative Names
B7.2; T lymphocyte activation antigen CD86; Activation B7-2 antigen; B72; B7-2; CTLA-4 counter-receptor B7.2
UniProt
P42081
Immunogen
The antibody was raised by immunising mice using the ARH-77 human B-lymphoblastoid cell line. Antibody BU63 was validated to recognise CD86.
Application Notes
Antibody First Published
Engel et al. The B7-2 (B70) Costimulatory Molecule Expressed by Monocytes and Activated B Lymphocytes Is the CD86 Differentiation Antigen Blood, Vol 84, No 5 (September l), 1994： pp 1402-1407 PMID：7520767
Note on publication
Showed that CD86 and B2-7 protein are the same molecule. BU63 was used to validate this by showing binding to CD86. These authors did not produce BU63 for their experiments, it was provided by Dr D. L. Hardie at the Fifth International Workshop on Human Leukocyte Differentiation.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
