Cat. No.
MABL-1147
Application
IP, WB, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
C8/144B
From
Recombinant Antibody
Specificity
This antibody recognises alpha chain of human CD8 (CD8a). CD8 is a cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). CD8 is a T-cell co-receptor involved in the recognition of MHC class I molecules. It is present on cytotoxic/suppressor T lymphocytes.
Alternative Names
Leu2; CD8; CD8A; T-cell surface glycoprotein CD8 alpha chain; T- lymphocyte differentiation antigen T8/Leu-2; CD8a; p32
UniProt
P01732
Immunogen
This antibody was raised by immunising Balb/c mice with a synthetic peptide corresponding to the 13 C-terminal residues of the cytoplasmic domain of human CD8a.
Application Notes
Tis anti-CD8 antibody can be used to detect suppressor/cytotoxic T-cells and their neoplastic counterparts in tissue sections (van Gool et al., 2015). C8/144B has been extensively applied in multiple studies where the detection of CD8+ lymphocytes was needed, such as characterisation of various inflammatory diseases, cancer treatment etc. (Petukhova et al., 2010; Stover et al., 2018). For mature T- cells, presence of CD8 and CD4 is mutually exclusive, and thus using this anti-CD8 antibody with another one diected against CD4 might serve a way of distinguishing between different T-cell subpopulation.Furthermore, C8/144B antibody can be utilised in the identification of certain neoplasms when presence or absence of the anti-CD4 signal is of a diagnostic value. For example, in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed.
Antibody First Published
Mason et al. Immunohistological detection of human cytotoxic/suppressor T cells using antibodies to a CD8 peptide sequence. J Clin Pathol. 1992 Dec;45(12)：1084-8. PMID：1479035
Note on publication
This article describes generation and characterisation of C8/144B antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
