Cat. No.
MABL-1214
Application
IHC-F, ELISA
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
ACC4
From
Recombinant Antibody
Specificity
This antibody binds the citrullinated C1 epitope (amino acids 359 – 369 : ARGLTGRPGDA) as an α-chain peptide of the collagen type II and also shows binding affinity for the cyclic citrullinated filaggrin peptide. C1 peptide contains two arginines which are post translationally converted to citrulline. ACC4 was found to recognize the first citrulline at position 360. This antibody does not cross react with cross react with the respective non citrullinated variant of the α chain of the C1 epitope.
Alternative Names
ACPA; CII; Col II; COL2A1; citrullinated CII; citrullinated Col II; Col C II; citrullinated proteins; antibodies against citrullinated proteins; Collagen alpha-1(II) chain; Alpha-1 type II collagen; C1 epitope of citrullinated collagen type II as an a-chain peptide
UniProt
P02458
Immunogen
The original antibody was generated by immunizing a DBA/1 or (B10.Q ×DBA/1) F1 mouse with calcium-dependent peptidyl arginine deiminase (PAD4) treated helical CII peptides containing T and B cell epitopes emulsified in CFA.
Application Notes
Antibodies to citrulline-modified proteins have a high diagnostic value in rheumatoid arthritis (RA). The binding specificity of mouse IgG1 version of this antibody towards citrullinated protein was determined through ELISA. This antibody was also used in the immunohistochemical staining of inflamed synovial tissue in the vicinity of cartilage in paw samples obtained from arthritis induced mice. This antibody was also reported to mediate arthritis in mice. ACC4 does not bind cartilage and cannot induce arthritis, but it can enhance the development of arthritis. The antibody is arthritogenic in combination with antibody M2139. Infusion of ACC4 induces collagen antibody-induced arthritis (CAIA) in the presence of the anti-Col II antibody M2139 (PMID: 19204106).
Antibody First Published
Uysal et al. Structure and pathogenicity of antibodies specific for citrullinated collagen type II in experimental arthritis. J Exp Med. 2009 Feb 16;206(2):449-62 PMID:19204106
Note on publication
Describes the generation of antibodies against triple helical C1 epitope on collagen type II (CII) which have a high diagnostic value in rheumatoid arthritis (RA).
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
