Cat. No.
MABL-1215
Application
WB, IHC
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
AE3
From
Recombinant Antibody
Specificity
This antibody is specific for cytokeratin type 2.
Alternative Names
CK13; CK14; CK15; CK16; Cytokeratin 13; Cytokeratin 14; Cytokeratin 15; Cytokeratin 16
UniProt
Immunogen
The original antibody was raised by immunizing a mouse with human epidermal keratins from P3 x 63 Ag8 cells.
Application Notes
The original version of this antibody was used for immunohistochemistry on human corneas (Haug et al., 2013; PMID:22429721), on human sentinel lymph node tissue (Atinkaya et al., 2005; PMID:16000990), and on tissue from rats bearing mammary tumours (Esendagli et al., 2009; PMID:18987061), and on lichen amyloidosis and macular amyloidosis tissue (Apaydin et al., 2004; PMID:15096140). Further this antibody was used for a western blot on DE-E, DE-F and DE-SF cell line lysates (Chang et al., 2010; PMID:19748110). This antibody was used for immunofluorescence on breast cancer cell lines MCF7, MDA-MB-231, SKBR3, SUM159, and SUM1315 and a hepatocellular carcinoma cell line HepG2 (Balic et al., 2011; PMID:20861793).
Antibody First Published
Apaydin et al. Cytokeratin expression in lichen amyloidosus and macular amyloidosis J Eur Acad Dermatol Venereol. 2004 May;18(3):305-9. PMID:15096140
Note on publication
The original paper tries to understand the role of epidermal cells in the pathogenesis of lichen amyloidosis (LA) and macular amyloidosis (MA).
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
