Cat. No.
MABL-1217
Application
WB, IF, IHC
Isotype
Engineer antibody
Species Reactivity
canine, Human
Clone No.
C-04
From
Recombinant Antibody
Specificity
This antibody is specific for CK-18.
Alternative Names
KRT18; K18; CK-18; Keratin-18; Cytokeratin-18; Cell proliferation-inducing gene 46 protein; Keratin, type I cytoskeletal 18
UniProt
P05783
Immunogen
The original antibody was raised by immunizing a BALB/c mouse with keratin-enriched preparations from cultured A431 cells.
Application Notes
The original version of this antibody was used for immunohistochemistry on sections of teratoma derived from iPS cells (Shao et al., 2009; PMID:19238173), on rat endometrial cell sheets and the resected uteri (Kuramoto et al., 2018; PMID:29980256), on fresh samples from either tumours or normal tissues distant from tumours (Bártek et al., 1991; PMID:1716305), and on sebaceous epithelioma tissue from 2 dogs (Yoon and Park., 2016; PMID:27822240). To determine the specificity of this antibody immunofluorescence was performed on A431 cells and the HeLa cytoskeleton. Further a western blot was performed on filament-enriched preparations using this antibody (Bártek et al., 1991; PMID:1716305). This antibody was also used for immunofluorescence on Fresh IT specimens in a study on nose polyps (Yu et al., 2013; PMID:24033346).
Antibody First Published
Bártek et al. A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology J Pathol. 1991 Jul;164(3):215-24. PMID:1716305
Note on publication
The original paper describes A series of 14 new mouse monoclonal antibodies (MAbs) to keratins the data suggesting their potential value in the differential diagnosis of human tumours.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
