Cat. No.
MABL-1218
Application
Blocking, IP, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
5H7
From
Recombinant Antibody
Specificity
This antibody is specific for HLA class I histocompatibility antigen all different chains. Antigen- presenting major histocompatibility complex class I (MHCI) molecule. In complex with B2M/beta 2 microglobulin displays primarily viral and tumor-derived peptides on antigen-presenting cells for recognition by alpha-beta T cell receptor (TCR) on HLA-A-restricted CD8-positive T cells, guiding antigen-specific T cell immune response to eliminate infected or transformed cells.
Alternative Names
HLA class I histocompatibility antigen; HLA; Human leukocyte antigen
UniProt
P04439
Immunogen
BALB/c mice were immunized by a series of four to six monthly injections of human PBLs, human Jurkat tumor ceIls, or enriched T lymphocytes.
Application Notes
The biotinylated antibody was used for a blocking experiment in human PBLs (Smith et al, 1994; pmid:7913109). Flow cytometry was preformed on human PBMC using this antibody (Woodle et al, 1997; pmid:9036961). This antibody was used for immunoprecipitation on C1R and T2. HMy2.C1R (C1R) are HLA-A negative and -B35 low human B-lymphoblastoid cells, and 0.174 X CEMR (T2) are T-B lymphoblastoid hybrid cells that are missing a large region of the MHC, including the genes encoding TAP1 and TAP2 (Dangoria et al, 2002; pmid:11978783).
Antibody First Published
Smith et al. Inhibition of T cell activation by a monoclonal antibody reactive against the alpha 3 domain of human MHC class I molecules J Immunol. 1994 Aug 1;153(3):1054-67. PMID:7913109
Note on publication
In this paper the antibody was generated by immunizing mice with human PBLs, human jurkat tumor cells or enriched T lymphocytes
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
