Cat. No.
MABL-1219
Application
IP, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
TAG-OCS 1
From
Recombinant Antibody
Specificity
This antibody is specific for clathrin heavy chain 1. Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. Acts as component of the TACC3/ch- TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. Plays a role in early autophagosome formation.
Alternative Names
Clathrin heavy chain on chromosome 17; CLH-17
UniProt
Q00610
Immunogen
The original mouse antibody was raised by immunizing female BALB/c mice with lxlO6>isolated Aldefluor-positive IGROV1 cells suspended in monophosphoryl-lipid A and trehalose dicorynomycolate (MPL+TDM) adjuvant.
Application Notes
The mouse version of this antibody was tested by immunohistochemistry on IGROV1 cells to assess its binding to IGROV1 (WO2016118090). Flow cytometry was preformed on subpopulations of IGROV1 dectected by the mouse version of this antibody (WO2016118090). Additionally, to determine the specificity of the mouse version of this antibody, flow cytometry was preformed on ovarian cancer stem cells (WO2016118090). Finally, protein extracts from IGROV1 membranes were successfully immunoprecipitated using the mouse version of this clone (WO2016118090).
Antibody First Published
WO2016118090
Note on publication
Describes the generation and characterization of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
