Cat. No.
MABL-1228
Application
Functional Studies, IP, WB, ELISA, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
BZ194
From
Recombinant Antibody
Specificity
This antibody is specific for murine serpentine receptor mCMKLR1 (aka ChemR23, DEZ). mCMKLR1 is a novel protein possessing high homology with members of the chemoattractant receptor family, and binds the chemoattractant chemerin. Selective mCMKLR1 expression has been demonstrated by mouse macrophages ex vivo, and by dendritic cell precursors cultured in vitro.
Alternative Names
Chemerin receptor; CHEMERINR; chemokine receptor-like 1; Chemokine- like receptor 1; ChemR23; CMKLR1; DEZ; G-protein coupled receptor ChemR23; G-protein coupled receptor DEZ; orphan G-protein coupled receptor, Dez; Resolvin E1 receptor; RVER1; Gpcr27; MGC126105; MGC126106
UniProt
P97468
Immunogen
This antibody was raised by immunising Wistar Furth rats with the mCMKLR1 peptide/KLH conjugate. The immunizing amino-terminal mCMKLR1 peptide with the sequence NH2- DSGIYDDEYSDGFGYFVDLEEASPWC-COOH, corresponding to residues residues 8–32 of mCMKLR1, with a nonnative carboxyl-terminal cysteine to facilitate conjugation to keyhole limpet hemocyanin (KLH).
Application Notes
The anti-mCMKLR1 BZ194 generates a robust staining signal when used in flow cytometry applications with mCMKLR1+ cells (Zabel et al., 2006). This antibody, together with other mAbs directed against chemokine receptors, could be used to determine the leukocyte expression profile of receptors during homeostasis or inflammation, to elucidate the role of various receptors in coordinating the immune response and in leukocyte development, and to identify other proteins interacting with the chemokine receptor. In particular, this antibody has been used to evaluate the functional responses of mCMKLR1-expressing cells to chemerin (Zabel et al., 2006), to demonstrate the esssential role of natural killer cells in resolution of antigen-induced inflammation in mice (Anuforo et al., 2018), to indicate a potential role for chemerin and CMKLR1 in the regulation of inflammatory responses in the tumor microenvironment (Rama et al, 2011), to visualise immediate immune responses to pioneer metastatic cells in the lung (Headley et al., 2016), and to investigate the regulatory role of chemerin in lung tumorigenesis and cancer-associated inflammation (Unver et al, 2018).
Antibody First Published
Zabel et al. Chemokine-like receptor 1 expression by macrophages in vivo regulation by TGF-beta and TLR ligands. Exp Hematol. 2006 Aug;34(8):1106-14. PMID:16863918
Note on publication
Describe the original generation of this antibody and its subsequent use in FACS to assess mCMKLR1 expression by mouse leukocytes.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
