Cat. No.
MABL-1229
Application
in vitro, in vivo, SPR, ELISA, FC, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
Mab420
From
Recombinant Antibody
Specificity
This antibody is specific for human and cynomolgus monkey CNN1.
Alternative Names
IBP-10; Protein GIG1; Protein CYR61; IGF-binding protein 10; IGFBP-10; CCN family member 1; Cysteine-rich angiogenic inducer 61; Insulin-like growth factor-binding protein 10
UniProt
O00622
Immunogen
The original antibody was raised by immunizing a BALB/c or NMRI mouse with recombinant human CCN1 containing the single amino acid exchange E173D or F185L
Application Notes
The original antibody was used for flow cytometry on suspension adapted HEK293 cells. In a further flow cytometry experiment it was shown that the antibody also bound to cynomolgus monkey CNN1 This antibody was used for an ELISA on both human and mouse CNN1. The binding affinity of this antibody to human CNN1 was determined using surface plasmon resonance. It showed a Kd of 3.02 * 10^-8. This antibody was used for immunofluorescence on NIH 3T3 cells or suspension adapted HEK293 cells transfected with the expression plasmids driving the expression of the CCN1. It showed it could correctly label the CNN1 protein in vitro. This antibody was used in vivo on different tumor cell lines xenografted into a SCID-beige mice. It was effective for SKOV3 cells and Panc-1 cells but not MDA-MB-435 (US20120258112A1).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
