Cat. No.
MABL-1232
Application
WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Bovine, Goat, Guinea Pig, Marmoset, Monkey, Pig, Sheep, Human
Clone No.
LH7.2
From
Recombinant Antibody
Specificity
This antibody is specific for amino acids 252 to 361 of Collagen alpha-1(VII) chain.
Alternative Names
COL7A1; LC collagen; Long-chain collagen; collagen VII
UniProt
Q02388
Immunogen
The original antibody was raised by immunizing a mouse with 1% Nonidet P40 extract of trypsinized cultured human keratinocytes.
Application Notes
The original antibody was used for western blotting on overlapping recombinant type VII collagen fusion proteins and pepsinized human type VII (Lapiere et al., 1994; PMID:7530994), and on type VII collagen fusion proteins (Tanaka et al., 1994; PMID:7524608). Further this antibody was used for an ELISA on non-overlapping type VII collagen fusion proteins encompassing both non- collagenous amino- and carboxyl-terminal domains and the collagenous helical domain (Lapiere et al., 1994; PMID:7530994). This antibody was used for immunohistochemistry on normal adult human skin freshly obtained from two different sites (abdomen and arm) (Shimizu et al., 1990; PMID:2354110), on different human tumours (Wetzels et al., 1991; PMID:1867328), and on normal human tissue (Heagerty et al., 1986; PMID:3527251).
Antibody First Published
Heagerty et al. Identification of an epidermal basement membrane defect in recessive forms of dystrophic epidermolysis bullosa by LH 7:2 monoclonal antibody: use in diagnosis Br J Dermatol. 1986 Aug;115(2):125-31 PMID:3527251
Note on publication
Using indirect immunofluorescence the original paper found intense labelling with LH 7:2 at the epidermal basement membrane (EBM) of normal skin, and in skin samples from patients with simplex, junctional, dominantly inherited dystrophic and acquired forms of epidermolysis bullosa (EB), as well as bullous pemphigoid.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
