Cat. No.
MABL-1234
Application
in vivo, therapeutic, Block
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
AFA
From
Recombinant Antibody
Specificity
This antibody is specific for human collagen I, showing high affinity to the GDF sequence (in A2_DGDFY, part of the α2(I)C-telopeptide), suggesting it is a critical epitope.
Alternative Names
CTTR1; α2Ct; α2(I)C-telopeptide; C-terminal telopeptide of the α2 chain; Procollagen I
UniProt
Immunogen
The original antibody was produced by immunizing mice with a synthetic peptide (GGGYDFGYDGDFYRA) whose sequence corresponds to that of the C-terminal telopeptide of the α2 chain (α2Ct).
Application Notes
This antibody binds human collagen I and blocks the binding activity of its C-terminal telopeptide region (denoted as CTTR1) consisting of two α1(I)C-telopeptides (denoted as α1Ct) and one α2(I)C-telopeptide (denoted as α2Ct). The KD of this antibody and its Fab and scFv with native α2Ct present in procollagen I and with synthetic α2Ct were 663 pM, 268 nM, 21 pM, 57 nM, and 75nM for full-length AFA/procollagen I, Fab AFA antibody/procollagen I, full-length AFA antibody/synthetic α2Ct, Fab AFA antibody/synthetic α2Ct, and scFv/procollagen I, respectively (US20200048333A1). A chimeric IgG version of the original IgA version of this antibody was engineered (Fertala et al., 2013; PMID: 23586407). This antibody has been shown to be an effective therapeutic tool for blocking collagen fibril formation in various models (Fertala et al., 2017; PMID: 28972447) (Fertala et al., 2017; PMID: 23586407).
Antibody First Published
Chung et al. Collagen fibril formation. A new target to limit fibrosis J Biol Chem. 2008 Sep 19;283(38):25879-86. doi: 10.1074/jbc.M804272200 PMID:18650436
Note on publication
The original publication introduces a novel concept for reducing fibrotic deposits by inhibiting the self-assembly of collagen molecules into fibrils, utilizing monoclonal antibodies targeting the telopeptide region to block collagen interactions and demonstrating efficacy in reducing collagen fibrils in vitro and in organotypic constructs.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
