Anti-Collagenase type IV mAbs [3G11](MABL-1242)

MABL-1242

Immunoscintigraphy, in vivo, MTT assay, ELISA, IF, IHC

Engineer antibody

Mouse

3G11

Recombinant Antibody

This antibody is specific for type IV collagenase.

MMP2; MMP-2; MMP 2; Matrix metalloproteinase 2; 72 kDa type IV collagenase; 72 kDa gelatinase; Gelatinase A

P33434

The original antibody was generated by immunizing BALB/c mice with type IV collagenase.

The immunoreactivity of the original format of this antibody (mouse IgG1, κ) was measured via ELISA and was shown to decrease by approximately 10%-20% after cold iodination with 131- iodide. The (131)I-labeled version of this antibody was demonstrated to be stable in vitro for almost 72 h. The blood clearance pattern of (125)I-labeled 3G11 was defined as a two-compartment model, with a 7.2 h T1/2α and a 345.2 h T1/2β, and clear scintigraphic images of human lung carcinoma PG were obtained using (125)I-labeled 3G11 at 72 h, which further improved at 120 h (Dai et al., 2003; PMID: 14693044). Lidamycin (LDM) was conjugated to the Fab version of this antibody, and the resulting immunoconjugate maintained most of its immunoreactivity to both type IV collagenase and mouse hepatoma 22 cells, as shown by ELISA, and exhibited more potent cytotoxicity to hepatoma 22 cells than free LDM by MTT assay. Furthermore, when administered intravenously, the Fab'-LDM conjugate was proven to be more effective against the growth of subcutaneously transplanted hepatoma 22 in mice than free LDM in two experiment settings, increasing mice survival time (Fengqiang et al., 2004; PMID: 15382678). The scFv version of this antibody was constructed and used in various configurations for IF and in vivo imaging and therapeutic applications (Zhong et al., 2010; PMID: 20303650) (Kong et al., 2015; PMID: 25824464).

Dai et al. [Immunoscintigraphy of anti-type IV collagenase monoclonal antibody in nude mice bearing human lung cancer xenograft] Ai Zheng. 2003 Dec;22(12):1243-8.PMID:14693044

The original publication focuses on evaluating the tumor-specific distribution of the anti-type IV collagenase monoclonal antibody (mAb) 3G11 through radio imaging in tumor-bearing nude mice.

100 μg Purified antibody.

1 mg/ml.

Protein A affinity purified

PBS with 0.02% Proclin 300.

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.