Cat. No.
MABL-1247
Application
Blocking, functional assays, neutralizing, FC
Isotype
Engineer antibody
Species Reactivity
Primates, Human
Clone No.
5G9
From
Recombinant Antibody
Specificity
This antibody is specific for complement C3b-iC3b. C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Alternative Names
Complement C3; C3; C3 and PZP-like alpha-2-macroglobulin domain- containing protein 1; Complement C3 beta chain; C3-beta-c; C3bc; Complement C3 alpha chain; C3a anaphylatoxin; Acylation stimulating protein; ASP; C3adesArg; Complement C3b alpha' chain; Complement C3c alpha' chain fragment 1; Complement C3dg fragment; Complement C3g fragment; Complement C3d fragment; Complement C3f fragment; Complement C3c alpha' chain fragment 2
UniProt
P01024
Immunogen
The original version of this antibody was created by immunizing a mouse with complement C3b-iC3b.
Application Notes
This antibody has been shown to inhibit the CPC-mediated lysis of PNH erythrocytes in humans (Lindorfer et al, 2016; pmid:20068220). A CDC assay was preformed using this antibody on human Burkitt’s lymphoma cell lines, Daudi, Raji, Z138 mantle cell lymphoma cell lines and diffuse large B cell lymphoma cell line SU-DHL-4 (Beurskens et al, 2012; pmid:22368276). This antibody can also be used for flow cytometry and neutralizing applications.
Antibody First Published
Beurskens et al. Exhaustion of cytotoxic effector systems may limit monoclonal antibody-based immunotherapy in cancer patients J Immunol. 2012 Apr 1;188(7):3532-41. PMID:22368276
Note on publication
It describes the use of this antibody toghether with the RTX antibody to test whether it could increase the effectiveness in cancer treatment.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
