Cat. No.
MABL-1246
Application
ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Guinea Pig
Clone No.
MF9
From
Recombinant Antibody
Specificity
MF9 is highly specific for guinea pig C3, with no binding observed between MF9 and guinea pig C3a, rat C3 and human C3.
Alternative Names
C3
UniProt
P12387
Immunogen
A female New Zealand White rabbit was immunised with guinea pig C3 by three subsequent subcutaneous injections. The RNA was isolated from the spleen, a phage display library constructed and then used to produce soluble scFv fragments.
Application Notes
ELISA was used to confirm the specificity of MF9 for guinea pig C3, with MF9 displaying high reactivity for gp C3. Reactivity with guinea pig C3a, rat C3, and human C3 was not observed (Hawlisch et al, 2000). MF9 was used to stain a rejected transplanted liver in guinea pigs (Hawlisch et al, 2000).
Antibody First Published
Hawlisch et al, 2000. Guinea pig C3 specific rabbit single chain Fv antibodies from bone marrow, spleen and blood derived phage libraries J Immunol Methods. 2000 Mar 6;236(1-2):117- 31 PMID:10699585
Note on publication
Describes the original generation of MF9 and its characterisation through ELISA and IHC.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
