Cat. No.
MABL-1245
Application
ELISA;
Isotype
Engineer antibody
Species Reactivity
Guinea Pig
Clone No.
MA11
From
Recombinant Antibody
Specificity
MA11 is highly specific for guinea pig C3, with no binding observed between MA11 and guinea pig C3a, rat C3 and human C3.
Alternative Names
C3
UniProt
P12387
Immunogen
A female New Zealand White rabbit was immunised with guinea pig C3 by three subsequent subcutaneous injections. The RNA was isolated from the spleen, a phage display library constructed and then used to produce soluble scFv fragments.
Application Notes
ELISA was used to confirm the specificity of MA11 for guinea pig C3, with MA11 displaying high reactivity for gp C3. A higher signal intensity is observed with MA11 than with MF9. Reactivity with guinea pig C3a, rat C3, and human C3 was not observed (Hawlisch et al, 2000).
Antibody First Published
Hawlisch et al, 2000. Guinea pig C3 specific rabbit single chain Fv antibodies from bone marrow, spleen and blood derived phage libraries J Immunol Methods. 2000 Mar 6;236(1-2):117- 31 PMID:10699585
Note on publication
Describes the original generation of MA11 and its characterisation through ELISA.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
