Cat. No.
MABL-1244
Application
SPR, ELISA
Isotype
Engineer antibody
Species Reactivity
Guinea Pig
Clone No.
KF7
From
Recombinant Antibody
Specificity
KF7 is highly specific for guinea pig C3, with no binding observed between KF7 and guinea pig C3a, rat C3 and human C3.
Alternative Names
C3
UniProt
P12387
Immunogen
A female New Zealand White rabbit was immunised with guinea pig C3 by three subsequent subcutaneous injections. The RNA was isolated from bone marrow, a phage display library constructed and then used to produce soluble scFv fragments.
Application Notes
ELISA was used to confirm the specificity of KF7 for guinea pig C3, with KF7 displaying high reactivity for gp C3. Reactivity with guinea pig C3a, rat C3, and human C3 was not observed. KF7 has a higher signal intensity than MF9 and MA11 (Hawlisch et al, 2000). SPR was used to quantify the dissociation rate constant of KF7; KF7 has a dissociation rate constant of 1.6x10^-4 s^-1 (Hawlisch et al, 2000).
Antibody First Published
Hawlisch et al, 2000. Guinea pig C3 specific rabbit single chain Fv antibodies from bone marrow, spleen and blood derived phage libraries J Immunol Methods. 2000 Mar 6;236(1-2):117- 31 PMID:10699585
Note on publication
Describes the original generation of KF7 and its characterisation through ELISA and SPR.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
