Cat. No.
MABL-1256
Application
WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
I8/1
From
Recombinant Antibody
Specificity
I8/1 antibody recognizes human complement factor D. Complement factor D is a serine protease of the alternative pathway of complement activation.Factor D cleaves factor B bound to C3b, generating the alternative pathway C3 convertase C3bBb and releasing the Ba fragment.
Alternative Names
Adipsin; C3 convertase activator; Properdin factor D; CFD; factor D
UniProt
P00746
Immunogen
This antibody was raised by immunizing BALB/c mice with the purified complement factor D and by the subsequent generation of hybridomas.
Application Notes
I8/1 antibody can be used for the identification of the complement factor D from human serum and plasma. For instance, this antibody was utilized in the quantitation of the factor D levels in blood of patients with renal failure via a senstive ELISA procedure (Oppermann et al., 1991). It is particularly useful as factor D plasma levels positively correlate with plasma creatinine and the creatinine clearance in patients with chronic renal failure (Volanakis et al., 1985; Grubb et al., 1985).
Antibody First Published
Oppermann et al. Quantitation of components of the alternative pathway of complement (APC) by enzyme-linked immunosorbent assays. J Immunol Methods. 1990 Oct 19;133(2)：181-90. PMID：2146321
Note on publication
This article describes the generation and characterization of the I8/1 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
