Cat. No.
MABL-1257
Application
IHC-P, IP, RIA, WB, ELISA, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
OX-21
From
Recombinant Antibody
Specificity
This antibody is specific for human C3bINA, which is secreted into the serum.
Alternative Names
C3b/C4b inactivator; CFI; AHUS3; ARMD13; C3BINA; C3b-INA; FI; IF; KAF
UniProt
P05156
Immunogen
C3bINA was purified from outdated human plasma, and subcutaneously injected into DBA/2 x Balb/c F1 mice.
Application Notes
This antibody binds C3bINA, and consequently can be used to rapidly purify enzymically-active C3bINA from human plasma using affinity chromatography (Hsiung, 1982), permitting the molecular characterisation of C3bINA. This technique has a variety of benefits, as it allows the purification of serum proteins at low concentration and the extraction of high yields and active enzymes, as well as being time-efficient.
Antibody First Published
Hsiung et al. Purification of human C3b inactivator by monoclonal-antibody affinity chromatography. Biochem. J. (1982)203,293-298 PMID：7103942
Note on publication
Describes the original use of this antibody in monoclonal-antibody affinity chromatography to purify C3bINA.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
