Anti-Cortactin mAbs [4F11](MABL-1260)

MABL-1260

IP, WB, IF, IHC

Engineer antibody

Avian, Bovine, Hamster, Rat, Human, Mouse

4F11

Recombinant Antibody

This antibody is specific for cortactin in both birds and mammals. It recognizes 80 kDa and 85 kDa Cortactin in avian cells, and also recognizes 80 kDa protein in rodent and human cells. Cortactin contributes to the organization of the actin cytoskeleton and cell shape.

Src substrate protein p85; CTTN1; Cortactin; p80

Q01406

The mouse version of this antibody was generated by immunizing mice with a mixture of affinity-purified tyrosine phosphoproteins from chick embryo fibroblasts expressing activated pp60src, and recognizes Cortactin.

To characterise the binding of the mouse version of this protein to tyrosine- phosphorylated protein, immunoblotting on tyrosine-phosphorylated proteins from CEFs, NIH 3T3, Rat-1, HOS and 10W was performed. Furthermore, immunoprecipitation on the same samples was preformed. The antibody showed a reaction in both experiments and had cross-reactivity with the other species (Kanner et al, 1990; pmid:2110361). To confirm the specificity of the mouse version of this antibody, immunoprecipitation of cortactin was preformed using this antibody. Furthermore, the immunoprecipitated antibodies were western blotted with this antibody. The western blot showed bands corresponding to cortactin. Finally, invadopodia were immunostained for cortactin using this antibody and showed co- localization with antibody clone #170-10, which is also an anti-cortactin antibody (Bowden et al, 1999; pmid:10442635). The human small bowel was stained by immunohistochemistry using the mouse version of this antibody. It showed strong staining of the brush border and it was especially concentrated at the terminal web of the brush border microvilli (Wu and Montone, 1998; pmid:9742075). FAK(+/+) and FAK(-/-) mice cell lysates were western blotted using the mouse version of this antibody. It showed expression of the cortactin protein in both FAK(+/+) and FAK(-/-) cells (Ilić et al, 1995; pmid:7566154). A431 human epidermal carcinoma cells were lysed and were then immunoprecipitated using the mouse version of this antibody.Cell lysates from A431 cells were also immunoblotted using this antibody (Crostella et al, 2001; pmid:11439336). The mouse version of this antibody was used to determine subcellular localization of cortactin in cortactin overexpressing clonal cell lines. Furthermore western blotting was preformed on extracts of Ems1,Ems2, and Neo1 cells (Patel et al, 1998; pmid:9681820).

Kanner et al. Monoclonal antibodies to individual tyrosine-phosphorylated protein substrates of oncogene-encoded tyrosine kinases Proc Natl Acad Sci U S A. 1990 May;87(9):3328-32. PMID:2110361

To study transformation-relevant tyrosine kinase substrates, we have generated monoclonal antibodies to individual tyrosine phosphoproteins, including p130, p120, p110, and five additional phosphoproteins (p210, p125, p118, p85, and p185/p64). These antibodies detected several of the same tyrosine phosphoproteins in chicken embryo fibroblasts transformed by avian retroviruses Y73 and CT10, encoding the yes and crk oncogenes, respectively.

100 μg Purified antibody.

1 mg/ml.

Protein A affinity purified

PBS with 0.02% Proclin 300.

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.