Cat. No.
MABL-1261
Application
equilibrium dialysis, RIA, ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
5A4
From
Recombinant Antibody
Specificity
This antibody binds cortisol. It has also shown cross-reactivity to dexamethasone and prednisolone. This is due to a hydroxyl group bound to C11. Cortisol is a steroid hormone, in the glucocorticoid class of hormones. It is produced in many animals, mainly by the zona fasciculata of the adrenal cortex in the adrenal gland. It is produced in other tissues in lower quantities. It is released with a diurnal cycle and its release is increased in response to stress and low blood-glucose concentration. It functions to increase blood sugar through gluconeogenesis, to suppress the immune system, and to aid in the metabolism of fat, protein, and carbohydrates. It also decreases bone formation. This antibody has not shown any affinity for cortisol-21-hemisuccinate-BSA.
Alternative Names
Hydrocortisone; ChEBI：17650
UniProt
Immunogen
The original antibody was generated by immunizing mice with cortisol-7-CM, followed by cortisol-3-CMO. Both forms of cortisol were bound to BSA.
Application Notes
The specificity and affinity of this mouse version of this antibody was determined using equilibrium dialysis in dialysis cells fitted with a cellulose membrane of 3500 MW cutoff (Calvez et al, 1995; pmid：7898495). Further a radioimmunoassay was performed on I125-cortisol using the scFv version of this antibody (Calvez et al, 1995; pmid：7898495). The specificity of this antibody towards cortisol-3-CMO-BSA, cortisol, prednisolone and dexamethasone was also tested using equilibrium dialysis. ELISA was used to show that this clone was able to bind steroids conjugated to BSA through nonspecific hydrophobic interactions (PMID： 9820517).
Antibody First Published
Calvez et al. Paratope characterization by structural modelling of two anti- cortisol single-chain variable fragments produced in E. coli. Mol Immunol. 1995 Feb;32(3)：185-98.PMID：7898495
Note on publication
Two monoclonal antibodies (mAbs), 5A4 and 6D6, directed against cortisol, have been obtained; 6D6 is used in an assay kit for cortisol. The antibodies also recognize other, structurally related steroids present in the sample assayed.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
