Cat. No.
MABL-1262
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
CT#45
From
Recombinant Antibody
Specificity
This antibody binds cotinine. Cotinine is an alkaloid found in tobacco and is also the predominant metabolite of nicotine. It is used as a biomarker for exposure to tobacco smoke.
Alternative Names
486-56-6; ChEBI：68641; (S)-Cotinine; Cotinina; Cotininum
UniProt
Immunogen
The original antibody was generated by immunizing BALB/c mice with a cotinine–albumin conjugate.
Application Notes
This antibody was used in the development of an ELISA for determining urinary cotinine to monitor tobacco smoke exposure. This ELISA produced dose–response curves for cotinine ranging from 0.40–100 ng per assay and was able to discriminate nicotine, cotinine N-oxide, cotinine N- glucuronide (cross-reactivity was each 0.5%, taking cotinine as 100%), (3′R, 5′S)-3′-hydroxycotinine O- glucuronide (0.6%) and (R, S)-norcotinine (1.5%). Another major nicotine metabolite, (3′R, 5′S)-3′- hydroxycotinine, showed 8.4% cross-reactivity with this antibody (Kobayashi, 2011)
Antibody First Published
Kobayashi et al. A monoclonal antibody-based enzyme-linked immunosorbent assay for human urinary cotinine to monitor tobacco smoke exposure. Analytical Methods 2011, Issue 9.
Note on publication
Describes the use of this antibody to develop an ELISA to detect urinary cotinine.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
