Cat. No.
MABL-1290
Application
FACS, in vivo
Isotype
Engineer antibody
Species Reactivity
Monkey, Human
Clone No.
NB25B-17
From
Recombinant Antibody
Specificity
The antibody is specific for CTLA-4. CTLA-4 is a protein receptor that functions as an immune checkpoint and downregulates immune responses.
Alternative Names
Cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; CD152
UniProt
P16410
Immunogen
The original antibody was generated by immunizing alpaca with human CTLA-4, followed by antibody library construction and phage display-based selection.
Application Notes
The binding affinity of humanized VHH-Fc chimeric antibody to human CTLA-4 expressed in CHO cells was shown by FACS experiments. The blocking activity of the humanized VHH-Fc chimeric antibody to the binding of CTLA-4 to CD80 or CD86 was shown using FACS. The antitumor activity of the humanized antibody was demonstrated in vivo in animal models. Specifically, a tumor animal model was established by inoculating mice with human breast cancer cells MDA-MB-231 (ATCC) to induce breast cancer. Mice were grouped and administered with the humanized version of the antibody, and tumor volume in tumor-bearing animals was measured periodically in size. Results showed that the humanized version of the antibody was able to significantly reduce tumor volume in tumor-bearing mice (CN113121689).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
