Cat. No.
MABL-1291
Application
Blocking, WB, ELISA, FC, IF
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
1B8
From
Recombinant Antibody
Specificity
This antibody is specific for CTLA-4. CTLA-4 is an inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.
Alternative Names
Cytotoxic T-lymphocyte protein 4; Ctla4; Cytotoxic T-lymphocyte- associated antigen 4; CTLA-4; CD152; UC10-4FIO
UniProt
P09793
Immunogen
The antibody was generated by immunizing Armenian hamsters with the extracellular portion of murine CTLA-4 fused to a murine IgG2.
Application Notes
ELISA was preformed on murine CTLA-4lg to test the binding specificity of the anitbody (Walunas et al, 1994; pmid:7882171). For flow cytometry on A20, M12.41 (B cells), L cell (fibroblast), and P815 (mastocytoma) cells this antibody was used (Perkins et al, 1996; pmid:8666782). This antibody was used for blocking in mice. It prevented T-cell proliferation (Leach et al, 1996; pmid: 8596936). Western blot was preformed on LN cells harvested from SJL mice using this antibody (Aruna et al, 2005; pmid: 16014414). This antibody was used for flow cytometry on LN cells harvested from SJL mice (Aruna et al, 2005; pmid: 16014414). Mouse CD4+ CD25+ T cells were used for a flow cytomic experiment using this antibody (Valsazina et al, 2006; pmid: 16618776). Thymi from Tg4 CTLA-4WT and B10.PL mice were immunostained with this antibody (Verhagen et al, 2012; pmid: 23267099). LN cells from mice were immunostained with this antibody (Paas-Rozner et al, 2003; pmid: 12743364). Cultured CD4+CD25+ Tregs of mice were used in an flow cytometry experiment in which this antibody was used (Wang et al, 2010; pmid: 20843956).
Antibody First Published
Walunas et al. CTLA-4 can function as a negative regulator of T cell activation Immunity. 1994 Aug;1(5): 405-13. PMID: 7882171
Note on publication
Describes the generation of the antibody by immunizing Armenian hamsters with the extracellular portion of murine CTLA-4 fused to a murine IgG2.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
