Cat. No.
MABL-1302
Application
antagonist, Cytotoxicity assays, Depletion, in vivo, SPR
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
53Hu37
From
Recombinant Antibody
Specificity
This antibody is specific for human CXCR3, a receptor for the C-X-C chemokines CXCL9, CXCL10, and CXCL11. CXCR3 mediates the proliferation, survival, and angiogenic activity of human mesangial cells (HMC) via a heterotrimeric G-protein signaling pathway. The CXCR3 isoform 2 also regulates the proliferation, survival, and angiogenesis of microvascular endothelial cells (HMVEC) through a cAMP- mediated signaling mechanism. Dysregulation of CXCR3 signaling has been linked to several disorders, including type 1 diabetes mellitus and psoriasis.
Alternative Names
CD183; CXC-R3; CXCR-3; CKR-L2; IP-10 receptor; GPR9; C-X-C chemokine receptor type 3; G protein-coupled receptor 9; Interferon-inducible protein 10 receptor; clone 53.6
UniProt
P49682
Immunogen
The original antibody was generated by humanizing antibody clone "53".
Application Notes
This human IgG1 antibody was evaluated for its effector functions, including Antibody- Dependent Cell-Mediated Cytotoxicity (ADCC) assays, where it was tested with primary human NK cells and the NK9.2 cell line as effector cells against CHO cells expressing human CXCR3. The antibody demonstrated cytotoxicity against CXCR3-expressing target cells. Binding studies using a Biacore T200 revealed that 53Hu37 displayed differential binding affinities to human and mouse Fcγ receptors, which are important for ADCC and complement-dependent cytotoxicity (CDC). In vivo, the antibody was administered to cynomolgus monkeys, leading to a reduction in effector memory CD4+ and CD8+ T-cells, as well as CXCR3 staining in the spleen. Biochemical stability analyses showed that 53Hu37 maintained favorable stability under various stress conditions. The antibody also exhibited strong binding kinetics to recombinant human CXCR3 (KD = 0.957 nM) (US20180214542A1).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
