Cat. No.
MABL-1303
Application
FC, ELISA
Isotype
Engineer antibody
Species Reactivity
Marmoset, Human, Mouse
Clone No.
9C5
From
Recombinant Antibody
Specificity
This antibody strongly binds human, mouse and marmoset CXCR3. Epitope mapping revealed that this antibody binds a peptide (FSSSYDYGENESDSCCTSPPCP) which corresponds to amino acid position 23-44 of the human CXCR3.
Alternative Names
CD183; CXC-R3; CXCR-3; CKR-L2; IP-10 receptor; GPR9; C-X-C chemokine receptor type 3; G protein-coupled receptor 9; Interferon-inducible protein 10 receptor
UniProt
P49682
Immunogen
The original antibody was generated by immunizing a CXCR3* knockout mice with LI.2 cells (a murine pre-B cell line) were transfected with the mouse CXCR3 gene.
Application Notes
The binding of this antibody with CXCR3 from various species was assessed by flow cytometry. It was shown to recognize LI.2 cells transfected with mouse CXCR3 (mCXCR3) or human CXCR3 (hCXCR3), but not untransfected LI.2 cells or cells transfected with other chemokine receptors (i.e. mCXCR2) in flow cytometry. Epitope mapping of this antibody was performed using ELISA. To access the in vivo effect, various concentrations of the antibody were injected intraveneously in a mice. It was seen that the original 9C4, mouse IgG1 version, completely depleted CD4+CXCR3+, CD8+/CXCR3+ and B2207CXCR3+ cells at a concentration as low as 2 mg/kg (WO2014186842). Chimeric antibodies derived from 9C5 called 9C5-3MFc and 9C5-Fc-KO and humanized verion are also available on request.
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
