Cat. No.
MABL-1311
Application
IP, ELISA, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
I-3859
From
Recombinant Antibody
Specificity
This antibody binds the human CXCR4.
Alternative Names
CD184; CXC-R4; CXCR-4; FB22; Fusin; HM89; LCR1; LESTR; LAP-3; C-X-C chemokine receptor type 4; Leukocyte-derived seven transmembrane domain receptor; Lipopolysaccharide- associated protein 3; LPS-associated protein 3; NPYRL; Stromal cell-derived factor 1 receptor; SDF-1 receptor
UniProt
P61073
Immunogen
The original antibody was generated by immunizing BALB/c mice with recombinant NIH3T3- CXCR4 cells and/or peptides corresponding to CXCR4 extracellular N-term and loops.
Application Notes
The binding of this antibody to human CXCR4 was tested using ELISA. This antibody is capable of immunoprecipitating both CXCR4 monomers and dimers. I-3859 can also recognize CXCR4 present on NIH3T3-hCXCR4 transfected cells, MDA-MB-231,U937 promyelocytic cancer cells and Hela cervix cancer cells by flow cytometry. This antibody is also found to compete with other Anti-CXCR4 mAb 515H7 for binding to cell membrance as analyzed by flow cytometry. This antibody can be used for the immunohistochemical staining of tumor cells expressing CXCR4. It was used for the staining of RAMOS and KARPAS299 xenograft tumor cells (US20140170677).
Antibody First Published
US20140170677
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
