Cat. No.
MABL-1313
Application
crystallography, ELISA
Isotype
Engineer antibody
Species Reactivity
n/a
Clone No.
R454511
From
Recombinant Antibody
Specificity
R454511 binds to residues 1, 2, 3, 4, 5, 9 and 11 of CsA.
Alternative Names
CsA; ciclosporin A, cyclosporine A
UniProt
Immunogen
BALB/c mice were immunised with a D-Lys^8-Cs derivative coupled to a carrier protein.
Application Notes
Mutagenesis of Cs-derivatives identified that R454511 binds to residues 1, 2, 3, 4, 5, 9 and 11 of CsA (Quesniaux et al, 1990). In ELISA experimenets, R454511 was found to bind to Cs conjugated with both Thr^2 (Thr^2-Cs-BSA) and Lys^8 (D-Lys^8-Cs-BSA) (Quesniaux et al, 1990). The crystal structure of R454511 in complex with CsA has been obtained to 2.65 Å resolution (Altschuh et al, 1992).
Antibody First Published
Quesniaux et al, 1990. Monoclonal antibodies to cyclosporin are representative of the major antibody populations present in antisera of immunized mice. Mol Immunol. 1990 Mar;27(3):227-36. PMID:2342487
Note on publication
Describes the original generation of the antibody and its characterisation through ELISA.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
