Cat. No.
MABL-1319
Application
IP, WB, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
44.1
From
Recombinant Antibody
Specificity
The antibody recognises Cytochrome b-245 light chain (Epitope ATAGRF+GGPQVNPI).
Alternative Names
CYBA; flavocytochrome b; Cyt b; Cytochrome b(558) alpha chain; Cytochrome b558 subunit alpha; Neutrophil cytochrome b 22 kDa polypeptide; Superoxide-generating NADPH oxidase light chain subunit; p22 phagocyte B-cytochrome; p22-phox; p22phox
UniProt
P13498
Immunogen
Application Notes
The expression of p22phox from HEK-293 cells was confirmed by western blot with the antibody (Nakano et al., 2007; PMID: 17140397). The antibody detected p22phox by western blot analysis (Burritt et al., 1995; PMID: 7622517 and Taylor et al., 2004; PMID: 15585859). The antibody bound permeabilized neutrophils as shown by flow cytometric analysis. The antibody immunoprecipitated cytochrome b (Burritt et al., 1995; PMID: 7622517).
Antibody First Published
Taylor et al. Cloning, sequence analysis and confirmation of derived gene sequences for three epitope-mapped monoclonal antibodies against human phagocyte flavocytochrome b. Mol Immunol. 2007 Jan;44(4): 625-37. PMID:16563510
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
