Cat. No.
MABL-1325
Application
ICC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
R1D3
From
Recombinant Antibody
Specificity
The antibody is specific for cytochrome c. The antibody binds to a variety of oxidatively modified cyt c species, such as nitrated at Tyr74 or oxidized at Met80, which lose the sixth heme ligation (Fe-Met80); it does not bind to several cyt c phospho- and acetyl-mimetics.
Alternative Names
cyt c; Cytochrome c somatic
UniProt
P62897
Immunogen
Application Notes
The antibody was employed to identify alternative conformations of cyt c in cells under oxidant- or senescence-induced challenge as confirmed by immunocytochemistry (Tomasina et al., 2022; PMID： 36378644).
Antibody First Published
Tomasina et al. De novo sequencing and construction of a unique antibody for the recognition of alternative conformations of cytochrome c in cells Proc Natl Acad Sci U S A. 2022 Nov 22;119(47)：e2213432119. PMID：36378644
Note on publication
The paper describes the sequencing of the original antibody
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
